DNA and lipid bilayers: self-assembly and insertion
نویسندگان
چکیده
منابع مشابه
DNA and lipid bilayers: self-assembly and insertion.
DNA-lipid complexes are of biomedical importance as delivery vectors for gene therapy. To gain insight into the interactions of DNA with zwitterionic and cationic (dimyristoyltrimethylammonium propane (DMTAP)) lipids, we have used coarse-grained molecular dynamics simulations to study the self-assembly of DPPC and DPPC/DMTAP lipid bilayers in the presence of a DNA dodecamer. We observed the spo...
متن کاملSelf-assembly of filopodia-like structures on supported lipid bilayers.
Filopodia are finger-like protrusive structures, containing actin bundles. By incubating frog egg extracts with supported lipid bilayers containing phosphatidylinositol 4,5 bisphosphate, we have reconstituted the assembly of filopodia-like structures (FLSs). The actin assembles into parallel bundles, and known filopodial components localize to the tip and shaft. The filopodia tip complexes self...
متن کاملSelf-assembled DNA nanopores that span lipid bilayers.
DNA nanotechnology excels at rationally designing bottom-up structures that can functionally replicate naturally occurring proteins. Here we describe the design and generation of a stable DNA-based nanopore that structurally mimics the amphiphilic nature of protein pores and inserts into bilayers to support a steady transmembrane flow of ions. The pore carries an outer hydrophobic belt comprise...
متن کاملInstability Phenomena in Lipid Bilayers and Lipid-DNA Complexes
Lipid bilayers membranes, positioned between two solutions, are the barrier of the cell and its internal components (organelles). Membranes define the cell limit and, by allowing the existence of two different solutions in close proximity, enable their functions. Membranes participate in various biological processes such as cell-cell adhesion, controlled ions transfer, and signal transduction. ...
متن کاملPolyglutamine expansion in huntingtin increases its insertion into lipid bilayers.
An expanded polyglutamine (Q) tract (>37Q) in huntingtin (htt) causes Huntington disease. Htt associates with membranes and polyglutamine expansion in htt may alter membrane function in Huntington disease through a mechanism that is not known. Here we used differential scanning calorimetry to examine the effects of polyQ expansion in htt on its insertion into lipid bilayers. We prepared synthet...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of The Royal Society Interface
سال: 2008
ISSN: 1742-5689,1742-5662
DOI: 10.1098/rsif.2008.0239.focus